Development and Validation of New Rp-hplc Method for the Determination of Afloqualone in Human Plasma and Formulation
نویسندگان
چکیده
A simple, rapid and precise reverse phase high performance liquid chromatography method has been developed and validated for the determination of Afloqualone in tablet and serum. Chromatography was performed by Shimadzu Model LC-20 ATVP with Kromasil C-18 column 250 x 4.6 mm ID with 5 particle sizes and the column maintained at ambient temperature. The injection volume was 20l and the total run time was 6 min. The detection was carried out at 285 nm. The mobile phase consisted of Acetonitrile, 1% Acetic acid and Water (40:40:20 v/v). Prepared mobile phase was filtered through 0.45 membrane filter and sonicated. Sample solution was prepared by dissolving the drug in mobile phase and sonicated for 30 minutes. The mobile phase was delivered isocratically at a flow rate of 1ml/min.
منابع مشابه
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